Hi all I have a blastx between a transcriptome (DNA) and a protein database. I have placed some commands to give me the best ORF as a result and thus delete sequences for further analysis. My question is: It is possible to extract the sequence of the blastx where he made a match, that is, obtain the transcriptome sequence in the reading frame where he made a match. In NCBI this can be done easily, since it delivers the ORF and then one goes to that sequence that made match. Waiting for your response, thank you very much
PD: my objective in this case is to determine if it is actually present the protein in the transcriptome