I'm trying to create a custom file.genome for IGV, but I can't and don't know what's the error... According to IGV instructions (https://software.broadinstitute.org/software/igv/LoadGenome), I'm following these steps:
- I've downloaded whole genome fasta and have extracted only those regions coding for genes (Is There A Way To Download The Human Exome?).
- In IGV, I select the create option pointing out this modified fasta, a text file with cytobands and a gtf downloaded from Ensembl.
- Generated .genome is (extremelly?) heavy (~40Mb) and IGV crashes trying to opening it (CPU is totally overhelmed).
Any idea, please?? Thanks in advance!!