Question: Identify TF binding sites genomewide
0
gravatar for sumithrasank75
21 months ago by
United States
sumithrasank75130 wrote:

What is the best tool to use to get the genomic coordinates (as a BED file) of the binding sites of a transcription factor? I would like to do this genomewide.

next-gen sequence genome • 564 views
ADD COMMENTlink modified 21 months ago by Alex Reynolds29k • written 21 months ago by sumithrasank75130
3
gravatar for Alex Reynolds
21 months ago by
Alex Reynolds29k
Seattle, WA USA
Alex Reynolds29k wrote:

I don't know if it is the "best", but a popular tool is FIMO, from the MEME suite.

For command-line work, you provide the FASTA of your genome of interest and your TF model(s) in MEME format, e.g. from JASPAR, UniPROT, TRANSFAC, etc.

A 10^-4 or -5 threshold is probably reasonable for whole-genome work; a more stringent threshold will give you fewer binding sites.

ADD COMMENTlink modified 21 months ago • written 21 months ago by Alex Reynolds29k

I agree. This is an area that I have been working on recently, particularly both JASPAR and TRANSFAC.

ADD REPLYlink written 21 months ago by Kevin Blighe52k
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