Question: RNA seq analysis when many genes are differentially expressed
gravatar for mccormack
2.4 years ago by
United States
mccormack40 wrote:

I know that many, if not all, differential expression analyses of RNA seq data assume that the majority of genes are not changing expression. However, what if you have data that when a FDR cutoff of 0.05 is used there are 30%, 40% or even 50% of genes are differentially expressed ? What distortions in the results would be expected ?

Does it make any difference if there are only 5 conditions where this is so, but the analysis (DESeq2) was done with a total of 30 conditions (60 HTSeq files, or 30 with duplicates).

edger rna-seq cuffdiff deseq2 • 1.1k views
ADD COMMENTlink modified 2.4 years ago by Kevin Blighe63k • written 2.4 years ago by mccormack40

Read previous discussion at Too many differentially expressed genes in RNA-seq?, and chech the links I provided at my answer.

ADD REPLYlink written 2.4 years ago by h.mon30k
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