How to get pre-mRNA sequence for analysis using mutation information?
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6.1 years ago
ckchembio • 0

I have mutation in VCF format( chromosome, position, ref, alt...), and I want to analysis how the mutation will affect RNA folding and so on. As a gene may have several transcription, so how could I get correct (reasonable) pre-mRNA sequence for analysis?

genome gene rna splicing • 882 views
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