Question: Base pair order in sequencer vs in fastq
gravatar for DVA
9 days ago by
United States
DVA390 wrote:

For illumina platform, after the reads bind to the chip and bridge amplified, machine reads each read from top to bottom. I am wondering how does this top-bottom order transfer to fastq files. Does it become left to right? In anther word, (assuming index sequence shows in the naming line of fastq, not with the read.) the adaptor that is close to the bottom of chip, will be at the right end of each read (assume this adaptor gets sequenced).

Thank you very much.

sequencing • 101 views
ADD COMMENTlink modified 9 days ago by finswimmer930 • written 9 days ago by DVA390
gravatar for finswimmer
9 days ago by
finswimmer930 wrote:

Hello DVA,

yes, "top-to-bottom" becomes "left-to-right". The sequencing direction is always 5' to 3' and that's what you have also in your fastq files. You can also say, the first base you sequence is also the first base in your fastq file.

Here is also a nice picture about the sequence process.

fin swimmer

ADD COMMENTlink written 9 days ago by finswimmer930

Thank you for the confirmation!

ADD REPLYlink written 9 days ago by DVA390
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