Hello, I am working with Illumina hiseq 2000, only single reads. I got my sequences from the EBI database:https://www.ebi.ac.uk/ena/data/view/PRJEB15445
I'm planning on doing some further analysis and for that I need pair-end sequences. I need to artificially generate the reverse reading. I am bit confused because I've read in different resources that stated that the Reverse sequence is only the reverse and in others that it is the reverse-complement. I was wondering if someone knows if the second read is only the reverse or the reverse-complement?
Thank you so much,