Question: The chip-seq binding sites of POLR2B indicate the PoII pause there
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gravatar for limeng
4 months ago by
limeng0
limeng0 wrote:

I noticed chip-seq for POLR2B which is a core component of PoII, have a lot of binding sites in gene body? Is this because the PoII pause there? or since PoII is rapidly moving, why it have binding sites?

poii chip-seq • 218 views
ADD COMMENTlink modified 3 months ago by Carlo Yague4.1k • written 4 months ago by limeng0
1
gravatar for Carlo Yague
3 months ago by
Carlo Yague4.1k
Belgium
Carlo Yague4.1k wrote:

One possible explanation: Pol II pauses on introns.

ADD COMMENTlink written 3 months ago by Carlo Yague4.1k
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gravatar for prakash
3 months ago by
prakash120
prakash120 wrote:

During transcription, pol II elongates over gene body. When you perform ChIPseq, you will find pol II density over promoter as well as gene body. The ratio of pol II enrichment at promoter and gene body determines whether gene is transcribed or not.

ADD COMMENTlink written 3 months ago by prakash120
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