What is the minimum number of non-ribosomal reads that can be used for E. coli transcriptomics?

Question

[O.rka]

I have paired end Illumina NEXTSeq reads with 75 bp read-length with samples in ~triplicates (sometimes duplicates, sometimes I'll have 6). I was thinking 2 Million reads would be a good target and if there are a few replicates with high pearson correlation that have over 1 Million then that should be sufficient. Is this normal practice? My ultimate goal is to look at logFC profiles not necessarily for differentially expressed genes.

[GenoMax]

Standard recommendation via GenoHub.com.