Hello! I am a beginner at RNA seq analysis, I was hoping someone would point me in the direction of how I can take a data set (~50K genes in rows + 200 cells in columns, I have gene counts), perform a differential gene expression analysis (i don't know if this is needed for a PCA) and subsequently a PCA, allowing me to visualize using a tSNE plot (with something like rTsne).
(all this using R would be great)
I apologize in advance if I am asking redundant or senseless questions!!
(codes would be helpful)