Question: Why do some library layouts have two files when its a paired library layout?
0
gravatar for anikethsuresh
22 months ago by
anikethsuresh20 wrote:

I have noticed that the number of files to download are different based on the type of library layout. For example, the data here comprises of two files. Is that because it is a paired-end library layout and each file corresponds to the forward and backward strand? Or has the file just been broken up?

Paired end Sequencing

library layout • 585 views
ADD COMMENTlink written 22 months ago by anikethsuresh20
2
gravatar for Pierre Lindenbaum
22 months ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum125k wrote:

Is that because it is a paired-end library layout

yes, and both files should contain the same number of reads and the same read-names in the same order

ADD COMMENTlink modified 22 months ago • written 22 months ago by Pierre Lindenbaum125k

Same read-names as in?

ADD REPLYlink written 22 months ago by anikethsuresh20

Read1 of the reverse file should have the same name as Read1 from the forward file.

ADD REPLYlink written 22 months ago by WouterDeCoster42k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 958 users visited in the last hour