Question: Why do some library layouts have two files when its a paired library layout?
0
gravatar for anikethsuresh
2.5 years ago by
anikethsuresh20 wrote:

I have noticed that the number of files to download are different based on the type of library layout. For example, the data here comprises of two files. Is that because it is a paired-end library layout and each file corresponds to the forward and backward strand? Or has the file just been broken up?

Paired end Sequencing

library layout • 764 views
ADD COMMENTlink written 2.5 years ago by anikethsuresh20
2
gravatar for Pierre Lindenbaum
2.5 years ago by
France/Nantes/Institut du Thorax - INSERM UMR1087
Pierre Lindenbaum130k wrote:

Is that because it is a paired-end library layout

yes, and both files should contain the same number of reads and the same read-names in the same order

ADD COMMENTlink modified 2.5 years ago • written 2.5 years ago by Pierre Lindenbaum130k

Same read-names as in?

ADD REPLYlink written 2.5 years ago by anikethsuresh20

Read1 of the reverse file should have the same name as Read1 from the forward file.

ADD REPLYlink written 2.5 years ago by WouterDeCoster44k
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