I want to extract sense and antisense transcript from .bam file using NASTIseq. The input smat and asmat are produced by htseq-count which following the post here. M. K. 's post here After running
ZSpred<-NASTIpredict(rice_ZS$smat,rice_ZS$asmat, rice_ZS$pospairs, negpairs),
ZSpred$predictions are consisted of half of "-Inf" result. So I can't continue to the next step to classify these results using ROCR. Hope someone help me figure out what's happening and what to do.