Question: Gene calling and annotation for soil community
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gravatar for xuling2015
2.3 years ago by
xuling201520
United States
xuling201520 wrote:

Hi All,

I have a large dataset from soil community: shotgun metagenome sequencing, 10 sequencing lanes from hiseq2500 150PE, 80 samples in total from different treatment. I finished quality control and assembled with megahit. The final assembled contigs file is ~30G. I'd like to do gene-calling, annotation and taxonomy assignment now. As there are so many contigs and final file size is 30G, does anybody has suggestions about which softwares should I use?

Thanks in advance!

annotation gene calling • 670 views
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