Question: Gene calling and annotation for soil community
gravatar for xuling2015
2.3 years ago by
United States
xuling201520 wrote:

Hi All,

I have a large dataset from soil community: shotgun metagenome sequencing, 10 sequencing lanes from hiseq2500 150PE, 80 samples in total from different treatment. I finished quality control and assembled with megahit. The final assembled contigs file is ~30G. I'd like to do gene-calling, annotation and taxonomy assignment now. As there are so many contigs and final file size is 30G, does anybody has suggestions about which softwares should I use?

Thanks in advance!

annotation gene calling • 670 views
ADD COMMENTlink written 2.3 years ago by xuling201520
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1856 users visited in the last hour