I have a large dataset from soil community: shotgun metagenome sequencing, 10 sequencing lanes from hiseq2500 150PE, 80 samples in total from different treatment. I finished quality control and assembled with megahit. The final assembled contigs file is ~30G. I'd like to do gene-calling, annotation and taxonomy assignment now. As there are so many contigs and final file size is 30G, does anybody has suggestions about which softwares should I use?
Thanks in advance!