I plan on analysing the following RNA seq data: cca 30M reads/sample, 15 samples, human data. The aim is to do differential expression analysis. I plan to perform alignment with HISAT2 and I wonder if my computer (specifications below) suitable for this?
- Processor: Intel(R) Core(TM) i7-4790S CPU @ 3.20GHz, 3201 Mhz, 4 Core(s), 8 Logical Processor(s)
- Installed Physical Memory (RAM) 16,0 GB
- HDD: WD Blue PC Desktop Hard Drive (WDC WD10EZEX) - 1TB
- SSD: Crucial BX100 SSD (CT1000BX100SSD1) - 1TB
If not I, what would my best option be - additional RAM (how much?) on this computer, different alignment tool, cloud computing (like AWS), galaxy?
Any input or experience is helpful! Thanks, Nina