best CDS prediction tool for online annotation submission
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4.6 years ago
willnotburn ▴ 50

I would like to annotate a metagenomic assembly with KEGG using the GhostKOALA service. The assembly was produced with megahit and is in FASTA format. GhostKOALA requires amino acid .faa files as input.

Question: what's the best tool to translate the assembly? I'm thinking to use prodigal to detect CDS features and feed those to GhostKOALA. Is there anything out there that's better/faster/newer? I also have bins, and it would be nice to detect rRNA in those, too.

annotation fasta cds prodigal ghostkoala • 1.3k views

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