Question: sortMeRNA before or after adapters trimming ?
0
gravatar for debitboro
5 months ago by
debitboro90
Belgium
debitboro90 wrote:

Hi Biostars community,

Shall I use sortMeRNA before or after poly(A) tails removal and/or adapters trimming ?

Thanks

rna-seq sortmerna rrna • 188 views
ADD COMMENTlink modified 9 weeks ago by caggtaagtat290 • written 5 months ago by debitboro90
0
gravatar for caggtaagtat
9 weeks ago by
caggtaagtat290
caggtaagtat290 wrote:

If you are using it for rRNA removal, I would use it after trimming, since I basically maps the reads to the rRNA database and with trimming beforehand, the process should be improved.

However be aware, that sometimes it can happen, that a random blank line gets integraded into your data

ADD COMMENTlink written 9 weeks ago by caggtaagtat290
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1767 users visited in the last hour