Question: sortMeRNA before or after adapters trimming ?
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gravatar for debitboro
7 months ago by
debitboro110
Belgium
debitboro110 wrote:

Hi Biostars community,

Shall I use sortMeRNA before or after poly(A) tails removal and/or adapters trimming ?

Thanks

rna-seq sortmerna rrna • 255 views
ADD COMMENTlink modified 4 months ago by caggtaagtat380 • written 7 months ago by debitboro110
0
gravatar for caggtaagtat
4 months ago by
caggtaagtat380
caggtaagtat380 wrote:

If you are using it for rRNA removal, I would use it after trimming, since I basically maps the reads to the rRNA database and with trimming beforehand, the process should be improved.

However be aware, that sometimes it can happen, that a random blank line gets integraded into your data

ADD COMMENTlink written 4 months ago by caggtaagtat380
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