Question: sortMeRNA before or after adapters trimming ?
gravatar for debitboro
3 months ago by
debitboro80 wrote:

Hi Biostars community,

Shall I use sortMeRNA before or after poly(A) tails removal and/or adapters trimming ?


rna-seq sortmerna rrna • 109 views
ADD COMMENTlink modified 7 hours ago by caggtaagtat240 • written 3 months ago by debitboro80
gravatar for caggtaagtat
7 hours ago by
caggtaagtat240 wrote:

If you are using it for rRNA removal, I would use it after trimming, since I basically maps the reads to the rRNA database and with trimming beforehand, the process should be improved.

However be aware, that sometimes it can happen, that a random blank line gets integraded into your data

ADD COMMENTlink written 7 hours ago by caggtaagtat240
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