Question: sortMeRNA before or after adapters trimming ?
gravatar for debitboro
5 months ago by
debitboro90 wrote:

Hi Biostars community,

Shall I use sortMeRNA before or after poly(A) tails removal and/or adapters trimming ?


rna-seq sortmerna rrna • 188 views
ADD COMMENTlink modified 9 weeks ago by caggtaagtat290 • written 5 months ago by debitboro90
gravatar for caggtaagtat
9 weeks ago by
caggtaagtat290 wrote:

If you are using it for rRNA removal, I would use it after trimming, since I basically maps the reads to the rRNA database and with trimming beforehand, the process should be improved.

However be aware, that sometimes it can happen, that a random blank line gets integraded into your data

ADD COMMENTlink written 9 weeks ago by caggtaagtat290
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