Question: sortMeRNA before or after adapters trimming ?
0
gravatar for debitboro
3 months ago by
debitboro80
Belgium
debitboro80 wrote:

Hi Biostars community,

Shall I use sortMeRNA before or after poly(A) tails removal and/or adapters trimming ?

Thanks

rna-seq sortmerna rrna • 109 views
ADD COMMENTlink modified 7 hours ago by caggtaagtat240 • written 3 months ago by debitboro80
0
gravatar for caggtaagtat
7 hours ago by
caggtaagtat240
caggtaagtat240 wrote:

If you are using it for rRNA removal, I would use it after trimming, since I basically maps the reads to the rRNA database and with trimming beforehand, the process should be improved.

However be aware, that sometimes it can happen, that a random blank line gets integraded into your data

ADD COMMENTlink written 7 hours ago by caggtaagtat240
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1464 users visited in the last hour