This is what I want to do: 1. There are phenotypical differences between two sets of mouse strains (Group 1: FVB, AKR, SJL. Group 2: Balb/c, C57BL6, C3H, DBA1, DBA2, NOD). 2. We know that Group 1 phenotype is genetically dominant and non-sex-linked. 3. I want to compare snps, insertions, deletions and mnps between two groups. 4. Find regions that are different between these two sets of strains. 5. Goal is to find the gene or a region responsible which then be addressed experimentally [depending on getting funding].
My Questions are: 1. Is this a feasible approach? If not, why not? 2. If yes, how should I go about it? 3. If it is not a feasible approach, can someone suggest a way to do a similar analysis in silico? Even if it is a feasible approach, is there a better way?
Sorry, I can’t be more specific since we still have not published our work. Also, we can’t do more experiments since there is no more funding for this work. Thanks for Consideration Sincerely Samir