Making a temporary transition for NGS sequencing back to Sanger and need a little help with simulating test files. We are working on validating software for variant calling from Sanger sequencing data and are looking to simulate AB1 files. We are hoping to introduce known variants and validate both the software and the potential users. Are there any tools available for accurately Simulating AB1 files from a Fasta reference sequence. What considerations should be made for ensuring the most accurate simulation? Command Line options are welcome. Thanks in advance.