Question: cufflinks missing some annotations?
gravatar for Sharon
12 months ago by
Sharon420 wrote:

Hi Every one

Why I am having some annotations missing in cufflinks -g option

chr1    Cufflinks       transcript      679399  679585  1000    .       .       gene_id "CUFF.2"; transcript_id "CUFF.2.1"; FPKM "12.3670741574"; frac "1.000000"; conf_lo "5.199859"; conf_hi "19.534289"; cov "90.723687"; full_read_support "yes";

But I have others is annotated with the gene:

chr1    Cufflinks       exon    621096  622034  1000    -       .       gene_id "OR4F16"; transcript_id "NM_001005277_1"; exon_number "1"; FPKM "0.0586063181"; frac "1.000000"; conf_lo "0.000000"; conf_hi "0.201545"; cov "0.396735";

I need to convert this gtf to fasta format and use it for lncRNA pipeline, but this CUFF annotations is causing some problems. I am using the same genome and gtf in calling cufflinks format. I also use Salmon, but don't know if it can give me my assembled transcripts in gtf of fasta ?


transcripts rna-seq gtf • 371 views
ADD COMMENTlink written 12 months ago by Sharon420

Hi Sharon, can you please define better "some problems"? Have you tried using the ggfread utility? What has been the result of using it?

ADD REPLYlink written 12 months ago by Fabio Marroni2.1k

Hi Fabio

Thanks. I am using lncscore, it is a tool for lncRNA. it takes assembled transcripts in fasta format, so I convert my transcricpts.gtf to fasta using cufflinks gffread tool to input the transcripts.fa to lncscore.

But when the original transcript.gtf has "Cuff" id instead of a gene id or transcript id, it shows up in the transcript.fasta format with the same cuff code, and this cuff code is not recognized by lncscore and the later fails.

My goal is to detect novel lncRNA, so I am open to different pipelines suggestions too. I am new to this lncRNA stuff :)

ADD REPLYlink modified 12 months ago • written 12 months ago by Sharon420
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