Entering edit mode
6.0 years ago
elaineyang622
•
0
my code is :
java -jar ~/tools/picard/picard-2.8.2.jar FastqToSam \
F1=../S0_L004_R1_001.fastq.gz \
F2=../S0_L004_R2_001.fastq.gz \
O=fastq_to_bam.bam \
SM=S0_L004
in the log file ,I can't find any error,but fastq_to_bam.bam is 0 byte.
The contents of the log file are as follows:
[Tue Apr 24 15:26:28 CST 2018] picard.sam.FastqToSam FASTQ=../S0_L004_R1_001.fastq.gz FASTQ2=../S0_L004_R2_001.fastq.gz OUTPUT=fastq_to_bam.bam SAMPLE_NAME=S0_L004 USE_SEQUENTIAL_FASTQS=false READ_GROUP_NAME=A SORT_ORDER=queryname MIN_Q=0 MAX_Q=93 STRIP_UNPAIRED_MATE_NUMBER=false ALLOW_AND_IGNORE_EMPTY_LINES=false VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json
[Tue Apr 24 15:26:28 CST 2018] Executing as lijie@node6 on Linux 2.6.32-504.el6.x86_64 amd64;
Java HotSpot(TM) 64-Bit Server VM 1.8.0_77-b03; Picard version: 2.8.2-SNAPSHOT
INFO 2018-04-24 15:26:29 FastqToSam Auto-detected quality format as: Standard.
INFO 2018-04-24 15:26:39 FastqToSam Processed 1,000,000 records. Elapsed time: 00:00:09s. Time for last 1,000,000: 9s. Last read position: * / *
INFO 2018-04-24 15:26:50 FastqToSam Processed 2,000,000 records. Elapsed time: 00:00:21s. Time for last 1,000,000: 11s. Last read position: * / *
......
......
......
So, what's wrong?
I see you are tagging "alignment" but FastqToSam is not an aligner. What are you trying to achieve?
we just see the first lines of the logs , what the final message ?
I just want to translate the fastq file into .bam ,and the fastq file is raw data,I'm not align to ref_genome
I'm having the same error and my final message is below, the file created is 0 bytes.
EDIT: I'm also getting these error codes, maybe they are related? I'm using a wrapper to submit all my jobs at once for about 100 files. I think the problem is with the input files, but I don't know if it's with the file themselves or because I am accessing files a directory above the script.