Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Tutorials
Tools
Jobs
Forum
Planet
All »
Home
Log In
Question: Difference between Allele frequency spectrum and Joint allele frequency spectrum
3
gravatar for beausoleilmo
3 months ago by
beausoleilmo • 190
McGill University
beausoleilmo • 190 wrote:

From what I understood, allele frequency spectrum is everything that concerns the frequency of all of the alleles studied in a population. It can be estimated with Tajima's D, theta, etc.

But how is it different from the joint allele frequency spectrum?

allele population structure • 236 views
ADD COMMENT • link •
modified 3 months ago by Alice • 230 • written 3 months ago by beausoleilmo • 190
4
gravatar for Alice
3 months ago by
Alice • 230
USA
Alice • 230 wrote:

Joint allele frequency spectrum is allele frequency distribution calculated between two\three populations. Imagine a 2D plot, where on x axis is AFS of population 1 and on y axis is AFS in population 2. For example, you can plot derived allele counts in Yoruba versus Central European population. Let's say, you count allele A x times in Yoruba and y times in Central Europe, then you plot it on 2D space. For three populations it will be a 3D plot. Upscaling this to a higher number of populations is computationally very hard. Joint AFS is informative about migrations and other parameters, because changes in jAFS will reflect changes in demography.

Take a look at this paper by Gutenkunst et al http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1000695

ADD COMMENT • link written 3 months ago by Alice • 230

Cool! That's great! But if I read the axis on the graph, it's from 0 to 20 which makes 21 comparisons.

we projected the data down to 20 sampled chromosomes per population

Where is the 21th comparison coming from? Also, is JAFS only on chromosomes or it can be on SNPs?

In addition if you have 2 populations, it's actually a 3D graphs (the elevation is the colour). Also, usually, the colour is the number of SNPs between two chromosomes. Can it be something else?

ADD REPLY • link modified 3 months ago • written 3 months ago by beausoleilmo • 190
1

My explanation is very simplified, I just made it to be intuitive. Furthermore, I guess these numbers may be log-transformed for plotting, and I don't remember exactly what they did for these figures. AFS is estimated from allele counts, i.e. how many times you see a SNP in a population. 20 chromosomes means there were 20 haplotypes, which can mean, for example, 10 imaginary individuals with a pair of chromosomes in each. I suggest reading this paper, it goes into details about allele counts, AFS and related concepts https://www.nature.com/articles/nrg4005

ADD REPLY • link written 3 months ago by Alice • 230
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1498 users visited in the last hour