which sequence file should be transcripts in full-length transcriptome analysis
Entering edit mode
3.1 years ago
fanglujing ▴ 60

Hi, I haven't analysis Pacbio sequence data before. Now, I have some results form pacbio full-length transcriptome data. I want to get transcripts sequence from these results for next analysis. I used polished consensus high quality sequences in cluster step results. But I wander whether this is correct because I found there was only about 9,000 sequences left after I remove the redundant sequence. which result should I use as the last transcripts? I also have the NGS sequences, so should I align NGS sequences to FLNC sequence to correct pacbio sequence or I just use the cluster result? what's the difference between use NGS sequence to correct pacbio sequence and cluster sequences? sorry for my English, and many thanks

full-length transcriptome Pacbio transcripts • 668 views

Login before adding your answer.

Traffic: 1096 users visited in the last hour
Help About
Access RSS

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6