which sequence file should be transcripts in full-length transcriptome analysis
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3.1 years ago
fanglujing ▴ 60

Hi, I haven't analysis Pacbio sequence data before. Now, I have some results form pacbio full-length transcriptome data. I want to get transcripts sequence from these results for next analysis. I used polished consensus high quality sequences in cluster step results. But I wander whether this is correct because I found there was only about 9,000 sequences left after I remove the redundant sequence. which result should I use as the last transcripts? I also have the NGS sequences, so should I align NGS sequences to FLNC sequence to correct pacbio sequence or I just use the cluster result? what's the difference between use NGS sequence to correct pacbio sequence and cluster sequences? sorry for my English, and many thanks

full-length transcriptome Pacbio transcripts • 668 views
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