coverage of sequencing data before downloading from SRA toolkit.

Question

[shuksi1984]

How can I check the coverage of sequencing data before downloading from SRA toolkit?

[Ram]

Hello shuksi1984!

Give us a little more context please, and please note that single sentence questions are not recommended, as mentioned here: C: Good quality whole genome human data source

Answer 1

[h.mon]

If you read the paper (provided there is one) describing the data. Or you could follow the suggestion from this post:

http://seqanswers.com/forums/showthread.php?p=189789

And calculate the expected theoretical coverage according to the Lander/Waterman equation:

C = L x N / G

Where L = read length; N = read number; and G = Genome size