Hello all, I would like to do DEG analysis on my count matrix using DESEQ2 package in R. My reads are in Ensembl Transcript ID and I know that I need to convert them to gene ID and sum up the reads belonging to the same gene before DEG, I know the package tximport for kalisto files but I have never done such directly from count matrix. Does anyone have some experience to share that how can I proceed?
Thanks a lot! p.s. the species is mmusculus (mouse)