Question: Per base sequence quality and per base N content
gravatar for misterie
2.5 years ago by
misterie90 wrote:

Hi, I have one incomprehensibleness with my FastQC's report. Usually N's have quality=2, so if our N content in e.g 99bp = 35% we should observe these on the first plot.

I attached printscreens from my FastQC and I have a question:

if on 99bp N's content >30%, and quality of N =2, why I cannot notice fewer value of lower whisker on the 1st plot? These N's should underestimate quality value in these position. enter image description here

enter image description here

fastqc quality • 1.2k views
ADD COMMENTlink written 2.5 years ago by misterie90
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1224 users visited in the last hour