Hi I'm trying to analyze bacterial RNA-seq data. To conduct RNAseq, I have annotated file format(gff3) described below
Modified gff3 format I have is like this:
Sequence_Name . CDS StartPosition EndPosition
But I want to generate gff3 format like this:
Sequence_Name . gene StartPosition EndPosition Sequence_Name . CDS StartPosition EndPosition
When utilizing RNAseq called Cuffdiff, I couldn't get gene name at gene column Is there any way to get gff3 format having two lines for each gene?
Thank you in advance.