Question: Extracting upstream and Downstream of a Gene based on different intervals
0
gravatar for always_learning
11 months ago by
Doha, Qatar
always_learning960 wrote:

Hi All,

I want to extract the regions for the few genes in upstream and downstream based on certain intervals for example in the range of 0-50, 50-5000, 5000-50000 basepair. What could be the best way to do that? One way I could think off is to make a bed files for these intervals separately and then search it using tools. Is there any other smarter way to do this?

Thanks

upstream intervals • 878 views
ADD COMMENTlink modified 11 months ago by sacha1.7k • written 11 months ago by always_learning960

You are on the right track. The general approach is described here: retrieving sequences of a upstream and downstream of a coordinate for hg19

ADD REPLYlink modified 11 months ago • written 11 months ago by genomax67k

To do something like this in the past, I began by retrieving the TSS locations for all genes from Ensembl Biomart. I then wrote a simple AWK command like the one in the link provided by @genomax to get exactly what I want, using the TSS positions as the starting location.

EDIT: With sachas answer below you now have the 2 main ways to do this. Both begin with getting the coordinates of your gene and then selecting what you want from a reference file via an scripted command (e.g. AWK) or a bedtools function.

ADD REPLYlink modified 11 months ago • written 11 months ago by YaGalbi1.4k

Thanks !!

How will I extract something like interval 50-5000 BPs region from a gene?

ADD REPLYlink written 11 months ago by always_learning960
3
gravatar for sacha
11 months ago by
sacha1.7k
France
sacha1.7k wrote:
  • Create a bed file with your gene. ( using refSeq.txt for instance ) : gene.bed
  • Use bedtools slop to make regions larger according how many basepair you want : gene_slop.bed
  • Use bedtools intersect between gene.bed and gene_slop.bed to keep only upstream & downstream region
  • Use bedtools gefasta to extract sequence
ADD COMMENTlink written 11 months ago by sacha1.7k
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