how to judge the 10X genomics single cell data quality

Question

[biologo]

Dear all,

i am analysis 10X genomics single cell data these days, but i donot know the specific standard to filter the sample, like how many gene it detect, how many cell it should have? or what other factors i should notice.

Thanks for your help.

[Devon Ryan]

There's generally no strict threshold always applied to things, but rather you want to look at distributions of values and see if there are any outliers with aberrantly low values that then might hinder getting good results. As a general rule, don't blindly apply filtering criterion, look at the data yourself.