Entering edit mode
5.9 years ago
shenwei1376
•
0
How everyone,
I have recently got a RNASeq data. The mRNA level of my target gene is extremely low though the phenotype is very strong. All the change trend is consistent with qRT-PCR test, indicating the analysis pipeline should be fine. The knockdown is achieved through introducing shRNA and no off-target can be found through prediction. I am wondering why my mRNA is so lower for this gene?
It will be really appreciated if anyone can give me any idea!
Many thanks!
