Obtaining exon-intron and intron-exon reads
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5.9 years ago

We're interested in studying the number of reads that start at an exon and "spill" over into the subsequent intron or vice-versa.

We have aligned .bam files and annotation files for h38. What tools/software could we use to get the number of reads from the .bam file that span exon-intron / intron-exon junctions?

RNA-Seq next-gen • 1.8k views
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We have aligned .bam files

how. Which tool ?

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We used STAR to get the alignments.

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Past thread with answers from @Pierre and @Devon: Extracting Intron-Exon Reads from bam files

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