Question: Taxonomy assignment from draft genome
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gravatar for msobol
3 months ago by
msobol10
msobol10 wrote:

Hi,

I have two assembled and annotated draft genomes of non-model fungal species. Based on the previous 18S analysis, they were both found to be closely related to Penicillium.

Now that I have the genome, I wanted to assign taxonomy. What is your preferred way of assigning taxonomy from draft genome assemblies?

Thanks, Morgan S.

ADD COMMENTlink modified 3 months ago by Vijay Lakhujani3.0k • written 3 months ago by msobol10

It depends how accurate the results should be. If you want something ultra fast you could try MASH against pre-sketched RefSeq. The slow way is to detect homologs between closely related species (e.g. ribosomal proteins) and build a tree from a concatenated trimmed alignment with e.g. RAxML and/or PhyloBayes

ADD REPLYlink written 3 months ago by 5heikki7.6k
0
gravatar for Vijay Lakhujani
3 months ago by
Vijay Lakhujani3.0k
India
Vijay Lakhujani3.0k wrote:

You can give KAIJU a try. And use NCBI BLAST nr +euk - as above, but also including fungi and microbial eukaryotes option

Paper link is here

You can also use standalone version which could be downloaded here

ADD COMMENTlink written 3 months ago by Vijay Lakhujani3.0k
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