Question: In RSAT retrieve_Ensembl_seq do FASTA sequences of genes on reverse strands take this into account?
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gravatar for Monika515
6 months ago by
Monika5150
Monika5150 wrote:

I am retrieving some orthologous promoter regions relative to the mRNA, some of the reference genes are on the reverse strand. What I want to know is if the retrieved FASTA sequence is of the reverse strand and is it flipped 3' to 5' so that the gene code can be read left to right, or if the default is to extract the forward strand 5' to 3'.

Or do I need to get the reverse complement, or just reverse the sequence?

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