I have some samples of exome sequences. I need to check the exome capture efficiency using picard collectHsmetrcies for each sample. I have target interval list with me. but I do not have idea about the bait interval list? Should I create from the bam file -> bed file -> interval ? Or what else I should do to get this bait interval list ? I just want to sure before doing it for the accuracy in method.
I will appreciate all the suggestions