Question: miRNA with UMI - any papers showing this improves accuracy?
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gravatar for Richard
2.4 years ago by
Richard570
Canada
Richard570 wrote:

Hi Folks,

It makes sens to me that using UMIs in miRNA-Seq would allow a user to get more accurate estimates of miRNA expression given how the inserts are really short and PCR is involved.

Although I'm finding a number of software packages supporting the analysis of data containing UMI, I'm not finding any papers that show results comparing with-and-without UMI miRNA results with an evaluation of accuracy.

Perhaps I'm missing a paper, (please let me know if that is the case), but if there isn't one, are people all in for UMI use in miRNA, or are there reasons beyond extra analysis steps that suggest results are better without the use of UMIs?

mirna umi • 1.2k views
ADD COMMENTlink modified 21 months ago by Rituriya30 • written 2.4 years ago by Richard570
1

Without digging through literature, I can tell you I've seen papers showing that UMIs improve accuracy when you want to deduplicate reads in order to see real differences in expression. Otherwise you can't be sure in your analysis if duplicates are real, or just an artifact of PCR amplification. Sorry that I didn't provide a paper but hope it helps.

ADD REPLYlink written 2.4 years ago by goodez480

I agree with Richard. Even I am struggling to find papers talking with and without UMI-based miRNA results. I am getting papers which mostly talk about how single cell RNA-seq can be benefitted with UMI. Any links would help. Thanks, Rituriya.

ADD REPLYlink written 21 months ago by Rituriya30
0
gravatar for Rituriya
21 months ago by
Rituriya30
Canada
Rituriya30 wrote:

Finally I found one research article just after posted the comment. Here it is. UMI by QIAseq method indeed looks promising when compared to the existing methods.

ADD COMMENTlink written 21 months ago by Rituriya30
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