How to Metanalysis of affymetrix chip (Clariom S and U133 a2 )
0
0
Entering edit mode
5.9 years ago
Kritika ▴ 260

Hi All

I know this question is asked previously many time. But i am facing some different issues . I have CEL file of Clariom S and U133 a2 affymetirx chip. CDF file for Clariom S is not available. I want to do meta-analysis of this data. I tried VirtualArray but its not working in my system. Is there any way i can do normalization putting all together the data generated from both the chip.

I also have normalised value (obtain separately ) of both chip. But it giving me huge difference For example.

Gene     Cl1          U1        U2         U3      U4
ABC     2.0         -10       -20        -15     -17

This data is from same(The data is from any of this phases that is 1 2 3 4 phase because same sample is processed with Clariom S. But the exact phase is unfortunately unknown ) . Here Cl1 is clariom sample and U1-U4 is u133 samples. Also I do not have control Based on this data we need to design internal control samples This data is phase wise data for example Phase 1 , 2, 3,4 Four phase are from U133 chip. I want to know my Clariom data is falling in which phase . Sorry i can not reveal exact details of samples that what this phases mean

Any help will be high appreciated !

Clarioms Cross Normalisation • 1.1k views
ADD COMMENT

Login before adding your answer.

Traffic: 2705 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6