Entering edit mode
6.3 years ago
k.kathirvel93
▴
310
Hi EveryOne, I am trying to simulate RNA-seq illumina data for differential expression analysis. I have used FluxSimulator and Polyester, but I need a tool which we can tell to that, these genes should be highly expressed and this should be down regulated.
- Can i do this with Polyester ? If i can, help me how to do.
Suggest me some best tools for my objective.
Thanks in advance.
I have added/removed tags to keep the post relevant. +1 for that question!
What's the utility in dictating which genes are up-regulated? Such tools are mostly useful for testing program, when you don't really care exactly which genes are up/down-regulated.
Hi Devon, Yes i really care about tools only. Because am creating pipeline for specific disease RNA-seq data analysis. I have a list of genes which may up regulated and down regulated in control and diseased state. So i want to simulate RNA-seq data with specified up regulated and down regulated genes.
I'd just generate a simulation and then change the labels on the output, that'd be simpler.