Question: Mapping data normalizatio
0
gravatar for Arash
9 weeks ago by
Arash20
Iran, Islamic Republic Of
Arash20 wrote:

Hi,

I have a question about RNA seq data:

  1. How much the best standard deviation for the mapping data?
  2. How can i say my mapping data in RNA seq is normal distribution?
  3. In normalization of mapping data, what is the mean of the reference (Median Mean, median median,....)?
  4. In normalization of mapping data, , which methods for RNA seq is the best choice? Scaling or quantile or by totals?

Thank you!

rna-seq clc • 113 views
ADD COMMENTlink modified 9 weeks ago by h.mon18k • written 9 weeks ago by Arash20
1

Standard deviation for what? The insert size? Nothing even uses that any more (don't waste your time with inferior tools like CLC when you can use better platforms for free).

ADD REPLYlink written 9 weeks ago by Devon Ryan82k
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