Quick conceptual question over whether this experiment truly would reflect a repeated measure or whether samples are indeed independent.
I have a cell culture that is grown in 5 different flasks to produce 5 biological replicates. These are then split further into 10x 6-well plates (3 tech reps which are later pooled) and exposed to 5 doses (including control). Now, for each dose (e.g. 1ug/ml, 2, 3, 4, 5) I get 5 bioreps.
My question is: is dose 1ug/ml in biorep 1 independent from 2ug/ml biorep 1, given that they are grown on different well plates or does this really count as repeated measures?
If I can grow cells in a flask to represent biological replicates, then wouldn't growing them further in different well plates also represent biological independence?