Question: merge aligned read pairs that overlap - keep the bases that match the reference
0
gravatar for Richard
17 months ago by
Richard570
Canada
Richard570 wrote:

Hi all,

I have aligned paired-end genome data that was sequenced with 150bp reads. The average insert size is just 200bp so there are lots of read pairs with significant overlap of their alignments.

I see that there are lots of tools for merging fastq data and at least one that clips overlapping read alignments.

I'm looking for a tool that uses a conservative approach that when merging or clipping overlapped read alignments, chooses the bases that match the reference over bases with high sequencing quality.

thanks, RIchard

paired-end illumina • 490 views
ADD COMMENTlink written 17 months ago by Richard570
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1025 users visited in the last hour