Question: tag count and fold enrichment
0
gravatar for mikysyc2016
9 months ago by
mikysyc201630
mikysyc201630 wrote:

Hi, When use masc2 to do peak calling will get a excel file which include fold enrichment( compare to input group). When use homer, it has one step to give you a file which include normalized tag count and control tags.(HOMER to count tags in each peak)

  1. whether we still need to know tag count, if we know fold enrichment and FDR? what is the function of tag count for ChIP-seq analysis?
  2. From the homer file:

.

Peak1 normalized tag count : 147.6  control tags : 37.1
Fold Change vs Control: 8.92      Fold Change vs Local : 16.48

I do not understand why fold change not equal to 147.6/37.1 close to 4? Thanks!

chip-seq • 371 views
ADD COMMENTlink modified 9 months ago by ATpoint14k • written 9 months ago by mikysyc201630
1
gravatar for ATpoint
9 months ago by
ATpoint14k
Germany
ATpoint14k wrote:

Because this macs fold change or fold enrichment, according to the paper is:

Candidate peaks with p-values below a user-defined threshold p-value (default 10-5) are called, and the ratio between the ChIP-Seq tag count and ╬╗local is reported as the fold_enrichment.

What this means in detail, I never figured out. That is why I only use the MACS peaks as template to aggregate reads, and then proceed with other tools like DESeq2 for downstream analysis.

ADD COMMENTlink modified 9 months ago • written 9 months ago by ATpoint14k
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