Question: Volcano plot for mean fluorescence intensity (flow cytometry) data
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gravatar for jackarnestad
10 months ago by
jackarnestad0 wrote:

I want to make a volcano plot based on the log fold change between two classes of samples which I have flow cytometry data on (over 50 markers). Most of the tutorials for volcano plots for RNA seq start with differential expression, but they require raw count matrices rather than the equivalent normalized matrix I am starting with.

How would you suggest calculating log fold change and p value for this data? From there, plotting a volcano plot is easy.

Thanks, Jack

R • 344 views
ADD COMMENTlink written 10 months ago by jackarnestad0

What exactly does your data look like?

ADD REPLYlink written 10 months ago by Devon Ryan89k
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