Question: Best Software for analysis of Hi-C data
gravatar for h.long
2.4 years ago by
h.long40 wrote:


I am just getting started analyzing Hi-C data to call TADs. It seems that there is not yet a consensus on which software tools are best to make an analysis pipeline. I have so far tried Hicup (from Babraham) for alignments, homer for making heatmaps and have been trying to install Juicer which offers a full pipeline.

I am wondering if anyone has any advice on which software tools they use in their analysis workflow. I would be particularly interested advice as to which is the best TAD caller to use.

Thanks for your help!

sequencing next-gen • 1.2k views
ADD COMMENTlink modified 2.4 years ago by Devon Ryan97k • written 2.4 years ago by h.long40
gravatar for Devon Ryan
2.4 years ago by
Devon Ryan97k
Freiburg, Germany
Devon Ryan97k wrote:

I'll give the somewhat biased recommendation to try HiCExplorer. It will enable starting with fastq files and proceeding through heatmaps and further analysis.

ADD COMMENTlink written 2.4 years ago by Devon Ryan97k

Hi Devon,

Thanks for your suggestion. The original publication for HiCExplorer was done on drosophila but I assume it can also be used for high organisms such as humans and mice?


ADD REPLYlink written 2.4 years ago by h.long40

Sure, mammalian genomes work just fine. We like to use fruit fly for development because their genomes are small, so it's (A) much cheaper to sequence and (B) much quicker to process. But we commonly use mouse and human samples with HiCExplorer these days.

ADD REPLYlink written 2.4 years ago by Devon Ryan97k
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