annotate a gene

Question

[kityharo]

Hello! I need to annotate a gene from a mushroom sequence that is not well sequenced, can someone help me?

[GenoMax]

Please edit the original question and provide following additional information.

• Are you looking to annotate a single gene? Do you know what gene it is?
• What kind of data do you have in hand?
• Are there related genomes available in databases?

Answer 1

[Benn]

If the gene is not well sequenced, get a good sequence first (crap in is crap out). With a good sequence you can try blast (tblastx), and see if there are significant matches.