Question: qPCR Ct value to the genome copy number
0
gravatar for lokraj2003
2.4 years ago by
lokraj200390
lokraj200390 wrote:

Is there any package for converting qPCR Ct values to the genome copy number. I already have standards required to fit the curve.

Thanks.

pcr R • 2.6k views
ADD COMMENTlink modified 2.4 years ago by Kevin Blighe67k • written 2.4 years ago by lokraj200390
1
gravatar for Kevin Blighe
2.4 years ago by
Kevin Blighe67k
Republic of Ireland
Kevin Blighe67k wrote:

You could do the calculations following the ΔΔ Ct (delta delta Ct) method. You don't need a package to do this - one could easily code it. The final inferred copy number would be:

2^-ΔΔCt

A value of 1 from this would relate to copy number 2, i.e., normal copy number; a value of 0.5 would relate to copy number 1; a value of 2 would relate to copy number 4.

There is a lot of information online about this formula. Just use a search engine to search for that information.

Kevin

ADD COMMENTlink modified 2.4 years ago • written 2.4 years ago by Kevin Blighe67k

I didn't know about this. I will try to look for it.

Thanks

ADD REPLYlink written 2.4 years ago by lokraj200390

Okay, no problem! Just remember that, for this method, you will require a control DNA sample, and also Ct values for control targets, like, 'housekeeper' genes.

ADD REPLYlink modified 2.4 years ago • written 2.4 years ago by Kevin Blighe67k
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