Question: qPCR Ct value to the genome copy number
0
gravatar for lokraj2003
4 weeks ago by
lokraj20030
lokraj20030 wrote:

Is there any package for converting qPCR Ct values to the genome copy number. I already have standards required to fit the curve.

Thanks.

pcr R • 118 views
ADD COMMENTlink modified 4 weeks ago by Kevin Blighe24k • written 4 weeks ago by lokraj20030
1
gravatar for Kevin Blighe
4 weeks ago by
Kevin Blighe24k
USA / Europe / Brazil
Kevin Blighe24k wrote:

You could do the calculations following the ΔΔ Ct (delta delta Ct) method. You don't need a package to do this - one could easily code it. The final inferred copy number would be:

2^-ΔΔCt

A value of 1 from this would relate to copy number 2, i.e., normal copy number; a value of 0.5 would relate to copy number 1; a value of 2 would relate to copy number 4.

There is a lot of information online about this formula. Just use a search engine to search for that information.

Kevin

ADD COMMENTlink modified 4 weeks ago • written 4 weeks ago by Kevin Blighe24k

I didn't know about this. I will try to look for it.

Thanks

ADD REPLYlink written 4 weeks ago by lokraj20030

Okay, no problem! Just remember that, for this method, you will require a control DNA sample, and also Ct values for control targets, like, 'housekeeper' genes.

ADD REPLYlink modified 4 weeks ago • written 4 weeks ago by Kevin Blighe24k
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