I went hard on myself the first year of my MSc (studied ALL day every day for a whole year, in a foreign, cold, COLD country) and got really sick, but before that, took up this giant task of assembling a never-before- assembled genome of an extremophile. I know, I took it upon myself and now regretting it. I figured out once I have the draft genome (which back then, my naive self-thought would be a proper, submitting it to NCBI genome-genome), I would find some PUFA related genes and compare them with those found in other extremophiles and build a story about the adaptation mechanisms in my organism.
Long story short, I was bed-ridden for two years and have severe anxiety now. I feel really shy with my incredibly nice supervisor, I have some questions about building my thesis story though, wondering if someone can brain-storm with me a bit. I have a draft de-novo assembled genome. I know which genes to target, I reciprocal blasted them to check their identity, and based on literature, they are part of the Pufa pathway (Pufa genes are connected to adaptaion to extreme temperatures)..But now what? What next? I am aware that my experiment wasn't set up properly, I have no proof of connecting these genes with adaptation to an extreme environment unless I observe their expression, but is there a way I can make a good Masters thesis of the mess that is my situation?
One option is to obviously present the Genome characteristics, GC content comparisons etc but I still feel something is amiss.
Asking because I feel safe here :)