I have filtered potential lncRNAs from a RNA-seq experiment following the usual pipeline for lncRNA identification (filtering by length, coding potential, protein domains, annotation...). For the functional analysis I want to predict interactions of DE lncRNAs with proteins and other RNA species (miRNAs, mRNAs). The problem is that I get several isoforms from cufflinks/cuffcompare in many lncRNA. Which isoform sequences should I maintain for further analysis? The most expressed one for each gene? Or all of them, supposing that each isoform has its own function?
Question: LncRNA isoforms RNA-seq
20 months ago by
Martin B. • 10
Martin B. • 10 wrote:
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