Question: RNA-seq edgeR normalization
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GuriGuri1565 • 30 wrote:
I have a matrix. I will enter gene expression values one by one into linear model. if gene1 is input, gene expression values of sample1 ~ sample 10 are input. I wonder how to normalize raw read counts using edgeR and that I can use library size, norm.factors about each genes as a result of calcNormfactors( ).
linear model : y ~ covariate + gene expression value
gene1 gene2 ... gene24596
sample1 read counts
sample2
.
.
.
sample10
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modified 2.7 years ago
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Devon Ryan ♦ 98k
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written
2.7 years ago by
GuriGuri1565 • 30