RNA-seq edgeR normalization
1
0
Entering edit mode
4.0 years ago
GuriGuri1565 ▴ 30

I have a matrix. I will enter gene expression values one by one into linear model. if gene1 is input, gene expression values of sample1 ~ sample 10 are input. I wonder how to normalize raw read counts using edgeR and that I can use library size, norm.factors about each genes as a result of calcNormfactors( ).

linear model : y ~ covariate + gene expression value

            gene1        gene2 ... gene24596 
 sample1   read counts
 sample2
   .
   .
   .
 sample10
RNA-Seq edgeR normalization TMM • 1.1k views
ADD COMMENT
2
Entering edit mode
4.0 years ago

Do not reinvent the wheel, your knowledge of statistics is insufficient to do so.

Use edgeR as described in its vignette and DO NOT USE A LINEAR MODEL.

ADD COMMENT

Login before adding your answer.

Traffic: 2027 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6