I'm using a NEBNext Ultra-directional RNA Library Prep kit for an RNA-seq experiment. I've been using 12 cycles of PCR during the amplification step and it has been working great, but I realized I accidentally used 15 cycles instead of 12 on the last run. Does anyone know of a way to control for differences in PCR amplification once I get the reads back? I unfortunately can't afford to redo the library prep for these samples. Any help would be amazing!