Question: Validate interesting gene from a burden test and association studies (SkatO, etc)
0
gravatar for Sharon
2.0 years ago by
Sharon480
Sharon480 wrote:

Hi Everyone

If we test the cummulative effect of a group of variants in a gene (or a region) using burden test and association analysis stuff. If a gene is found very significant, should we validate all variants in the gene using sanger sequence? Doesn't seem reasonable, so what we can do to prove this finding? And also is there any further analyis we should do before any wet lab step? Like something more than pathways, genes functions,GO, etc.

Thanks

ADD COMMENTlink modified 2.0 years ago by WouterDeCoster44k • written 2.0 years ago by Sharon480
1

I have altered your title to make it more concise, and removed the bold text from your post. There is no need for that. I have also added more appropriate tags to your question. These are important because as such experts can easily find your question.

ADD REPLYlink modified 2.0 years ago • written 2.0 years ago by WouterDeCoster44k
3
gravatar for WouterDeCoster
2.0 years ago by
Belgium
WouterDeCoster44k wrote:

I believe it makes sense to validate a random subset of variants, to give you an idea what the overall false positive rate is of your experiment. An important next step would be to repeat the association in an independent population.

ADD COMMENTlink modified 2.0 years ago • written 2.0 years ago by WouterDeCoster44k

Thanks WouterDeCoster :)

ADD REPLYlink written 2.0 years ago by Sharon480
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