Question: Validate interesting gene from a burden test and association studies (SkatO, etc)
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gravatar for Sharon
13 months ago by
Sharon440
Sharon440 wrote:

Hi Everyone

If we test the cummulative effect of a group of variants in a gene (or a region) using burden test and association analysis stuff. If a gene is found very significant, should we validate all variants in the gene using sanger sequence? Doesn't seem reasonable, so what we can do to prove this finding? And also is there any further analyis we should do before any wet lab step? Like something more than pathways, genes functions,GO, etc.

Thanks

ADD COMMENTlink modified 13 months ago by WouterDeCoster40k • written 13 months ago by Sharon440
1

I have altered your title to make it more concise, and removed the bold text from your post. There is no need for that. I have also added more appropriate tags to your question. These are important because as such experts can easily find your question.

ADD REPLYlink modified 13 months ago • written 13 months ago by WouterDeCoster40k
3
gravatar for WouterDeCoster
13 months ago by
Belgium
WouterDeCoster40k wrote:

I believe it makes sense to validate a random subset of variants, to give you an idea what the overall false positive rate is of your experiment. An important next step would be to repeat the association in an independent population.

ADD COMMENTlink modified 13 months ago • written 13 months ago by WouterDeCoster40k

Thanks WouterDeCoster :)

ADD REPLYlink written 13 months ago by Sharon440
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