Can IGV tell me where to look?
0
0
Entering edit mode
5.8 years ago
francois ▴ 80

Hopefully that is a dumb question!

I have Nanopore MinION reads from a (dirty) PCR and I am looking at my hg38-alignment on IGV.

Can IGV somehow tell me where to look for reads/coverage? For example at the level of a chromosome. I do not understand how I am supposed to visually scan for reads/coverage.

igv nanopore minion • 1.5k views
ADD COMMENT
0
Entering edit mode

You need to zoom in enough (choose a chromosome) and then start looking for read outlines to show up. You could also run qualimap on your BAM file to get an overview of alignment stats which includes coverage graphs.

ADD REPLY
0
Entering edit mode

I think you should have a look at samtools view which will tell you where reads are. If that's too much information you could try bedtools genomecov

ADD REPLY

Login before adding your answer.

Traffic: 2618 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6